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Passive acoustic monitoring for bats has become a common method to determine species presence and activity levels. However, current acoustic methods are ineffective for monitoring species abundance at large summer colonies. We used synchronized acoustic and thermal-imaging data collected at 6 colonies of Myotis grisescens (Gray Bats) and found a significant positive relationship between acoustic energy and number of emerging bats. Our findings reinforce that acoustics have the potential to estimate population sizes of summer bat colonies. Additionally, we examined ultrasonic amplitude variance across 19 AudioMoth devices at 5 different gain settings and found significant differences among devices and settings. Further exploration into device variability and bat behavior are necessary to develop a robust model of population estimates using acoustic energy.more » « lessFree, publicly-accessible full text available April 17, 2026
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